Praha-Brno is one of the busiest intercity routes in Czechia, and it is a good place to learn how regional travel is put together. České dráhy runs an hourly service between Praha hlavní nádraží and Brno hlavní nádraží on the main line, with InterCity and Railjet-branded trains on the corridor.
Coaches run on the same city pair from Praha, ÚAN Florenc, and FlixBus is one of the operators listed on direct services. Since both modes are frequent, the useful question is not train or bus in the abstract, but which one fits your departure window and where you need to arrive.
That is why we publish the corridor as a board rather than a slogan: mode, station, journey range and transfer count sit side by side, so you can see which answer suits the trip in front of you. The same method works for the brno praga autobus direction, which is the return leg almost every visitor books separately.